Col4a1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC
Recommend Dilution	ELISA: 1/100-1/400. Immunofluorescence: 1/40-1/60. Immunohistochemistry on Paraffin Embedded Sections: 1/250-1/1000. Antigen retrieval is not necessary but may improve results, the recommended fixatives are AFA, 5-10% buffered formalin or Bouin's.
Reactivity	Mouse, Orang-Utan
Host	Rabbit
Clonality	Polyclonal
Immunogen	Collagen IV purified from Mouse EHS tumour
Specificity	Antibody BP975 is specific for Mouse collagen type IV. The following cross reactivities have been observed: Mouse Type IV: 100% Mouse Type III: < 0.1% Mouse Type II: < 0.1% Mouse Type I: < 0.1% Human Type V: < 0.1% Human Type IV: < 0.1% Mouse Fibronectin: < 0.1% Mouse Laminin: < 0.1%
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Purification	Antigen Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	collagen, type IV, alpha 1
Database Link	Entrez Gene 12826 Mouse UniProt ID P02463
Background	Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
Synonyms	COL4A1
Reference Data

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