Procollagen Type III Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R
Recommend Dilution	RIA: > 1/200. ELISA: 1/100-1/200 (OD ≥ 500). Immunofluorescence (indirect, 1/40 on Frozen tissues). Immunohistochemistry on Frozen and Paraffin Embedded Tissue Sections: 1/500. Pretreatment: After removing Paraffin pre-treat with 0.2% Hyaluronidase (approx. 300 U/mg) in TBS, 15 min at 37°C, then block unspecific binding with blocking serum or 3% BSA in TBS and perform blocking of endogen Peroxidase with 1% H2O2 in TBS, if necessary. Incubation Time: 60 min at room temperature or overnight at 2-8°C. Positive Control: Human or Bovine Skin and Liver.
Reactivity	Bovine, Human, Porcine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Human Procollagen type III C-terminal and N-terminal PIIICP separated.
Specificity	Human and Bovine Pro-Collagen Type III (100%). Human and Bovine Pro-Collagen Typ I and Collagen Typ I <0.1% (RIA at 1/200 dilution).
Formulation	PBS without BSA or preservatives State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.1 ml sterile distilled water.
Concentration	~1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type III collagen is an alpha1(III)-trimer, MW 95 kDa, which forms 67 nm cross-banded fibrils. Typically it can be observed in skin, cartilage and vitreous body.
Synonyms	Collagen, Pro-Collagen
Reference Data

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