Collagen I (COL1A1) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, R, WB
Recommend Dilution	RIA. ELISA: 1/200 (OD ≥ 500). Immunflourescence Assays: 1/80. Immunohistochemistry on Paraffin Sections: 1/200-1/600. Pretreatment: After de-waxing the tissue slices they are treated with 0.2% Hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8°C over night. Positive Control: Human skin.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified Collagen type I from Human skin.
Specificity	Human Collagen type I: 100% Collagen type II and IV: < 0.1% Collagen type III and V: < 1.0% Human Fibronectin: < 0.1% Cross-reactivity with cattle <1.0%, rat and chicken < 0.1% (RIA at a 1:500 dilution)
Formulation	Phosphate buffered solution, no BSA and preservative added. State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.1 ml distilled water.
Concentration	~1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Predicted Protein Size	136.5 kDa
Gene Name	collagen type I alpha 1
Database Link	Entrez Gene 1277 Human UniProt ID P02452
Background	Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.
Synonyms	OI4
Reference Data
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion

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