Dopamine Receptor D4 (DRD4) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommend Dilution	Immunohistochemistry /Immunocytochemistry: this antiserum has been found to stain specific cells in various regions of PLP fixed rat brain sections at a dilution of 1:10,000. This includes the glomeruli of the olfactory bulb, staining of cortex layer 4 and dendritic staining in cortex layers 2 and 3. In layer 5 of the cortex, apical dendrites of many neurons were stained. In the hippocampus, diffuse immunoreactivity was observed in the molecular layer of the dentate gyrus, with fine dots associated with many neurons in the granule cell layer. Western immunoblot: using whole rat brain homogenate resulted in a single wide band being detected at ~48-53 kD at 1:500 dilution. ELISA.
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	D4 (aa. 176-185) covalently attached to a carrier protein
Specificity	Specific for Dopamine D4 Receptor (a.a. 176-185). This antisera has been characterized by immunocytochemical, western immunoblot and ELISA techniques. Cross-reactivity: D4 Dopamine Receptor (176-185) 100% D4 Dopamine Receptor 60% D1 Dopamine Receptor (9-21) 0% D1 Dopamine Receptor 0% D2 Dopamine Receptor (272-282) 0% D2 Dopamine Receptor 0% D3 Dopamine Receptor (2-10) 0% D3 Dopamine Receptor 0% D5 Dopamine Receptor (23-35) 0% D5 Dopamine Receptor 0%
Formulation	State: Serum State: Lyophilized neat serum without preservatives
Reconstitution Method	Restore with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer for more dilute antisera.
Conjugation	Unconjugated
Storage Condition	Lyophilized: Store at 2-8°C (preferably in a desiccator). Reconstituted: Aliquot and store at -20°C. Avoid repeated freezing and thawing.
Gene Name	dopamine receptor D4
Database Link	Entrez Gene 1815 Human UniProt ID P21917
Background	This gene encodes the D4 subtype of the dopamine receptor. The D4 subtype is a G-protein coupled receptor which inhibits adenylyl cyclase. It is a target for drugs which treat schizophrenia and Parkinson disease. Mutations in this gene have been associated with various behavioral phenotypes, including autonomic nervous system dysfunction, attention deficit/hyperactivity disorder, and the personality trait of novelty seeking. This gene contains a polymorphic number (2-10 copies) of tandem 48 nt repeats; the sequence shown contains four repeats.DRD4 expression has been reported in various regions of the brain as well as in adrenal gland, artery, eye, heart, kidney, placenta, spinal cord, testis, and vas deferens.
Synonyms	DRD4, D(2C) dopamine receptor
Reference Data

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