Collagen I (COL1A1) (+ type II, III, IV, and V) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC
Recommend Dilution	ELISA (1/2000-1/8000). Immunoflourescence (1/10-1/40). Immunohistochemistry on Frozen Sections (1/10-1/40).
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	A mixture of human collagen 1-5 from human placenta.
Specificity	This antibody detects exhibits the following reactivities in ELISA both native and denaturated Collagen types: Collagen types 1 and 3: 100%. Collagen types 4 and 5: 90%. Collagen type 2: 80%. Human Fibronectin, Albumin and Immunoglobulins all < 0.5%.
Formulation	PBS, pH 7.5 containing 0.1% Mannitol and 0.1% Dextran and 0.09% Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction.
Concentration	lot specific
Purification	Affinity Chromatography on immobilized collagen types I-V
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	collagen type I alpha 1
Database Link	Entrez Gene 1277 Human UniProt ID P02452
Background	Collagen I is a member of group I collagen (fibrillar forming collagen), it forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. Collagen II is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. High expression of isoform 2 in juvenile chondrocyte and low in fetal chondrocyte. Collagen III occurs in most soft connective tissues along with collagen I. Collagen IV is the major structural component of glomerular basement membranes, forming a Chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Collagen V is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Collagen V binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Synonyms	COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion

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