EMA (MUC1) Mouse Monoclonal Antibody [Clone ID: VU-3C6]

Specifications

Product Data
Clone Name	VU-3C6
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry.  Western Blot: 1/200-1/1000. Immunoprecipitation. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: Requires antigen retrieval using heat treatment (Sodium citrate buffer pH 6.0) prior to staining of paraffin sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human breast cancer cell line ZR-75-1.
Specificity	The dominant epitope recognized by this antibody is the 12-mer GVTSAPDTRPAP of the Mucin 1 tandem repeat.   This antibody has been tested on Frozen Human ovarian carcinoma tissue sections and paraffin embedded sections from normal breast tissue. It also reacts weakly with sections of small intestine and colon.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mucin 1, cell surface associated
Database Link	Entrez Gene 4582 Human UniProt ID P15941
Background	MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells.
Synonyms	MUC-1, PEMT, Episialin, EMA, H23AG, PUM, DF3, CA 15-3
Reference Data

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