CTNNA3 (164-177) Mouse Monoclonal Antibody [Clone ID: 892_24D2S]

Specifications

Product Data
Clone Name	892_24D2S
Applications	FC, IF, IHC, IP, WB
Recommend Dilution	Western blot. Flow Cytometry. Immunocytochemistry on Acetone-Fixed Cells. Immunohistochemistry on Frozen and Paraffin Sections. Immunoprecipitation. Recommended dilutions: 1/25-1/50 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/1000 for immunoblotting applications.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to amino acids 164-177 of the Human αT-Catenin protein including an additional N-terminal cysteine coupled to KLH
Specificity	The 892_24D2S antibody against αT-Catenin does not show any cross reaction with αE-Catenin or αN-Catenin in western blot analysis. This 892_24D2S antibody reacts with an epitope located between residues 164-177 of the Human αT-Catenin protein.
Formulation	PBS with 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Gene Name	catenin alpha 3
Database Link	Entrez Gene 29119 Human UniProt ID Q9UI47
Background	Catenins are proteins that connect cadherin/β- catenin cell adhesion complexes to the actin cytoskeleton. Although originally discovered in testis, αT-catenin is expressed in other tissues, the highest levels being observed in heart. Immunohistochemical analysis showed human αTcatenin localization at intercalated discs of cardiomyocytes and in peritubular myoid cells of testis. It is proven that αT-catenin can functionally restore cell-cell adhesion in colon cancer cells lacking α-catenins. This indicates that this protein is involved in the formation of specific cell-cell contacts in specific types of muscle cells. The encoded protein has about the same predicted size (100 kDa) as other α-catenins to which it shows an overall amino acid identity of 57%. In cells transfected with αT-catenin cDNA, interaction with β-catenin was demonstrated by coimmunoprecipitation.
Synonyms	Alpha T-catenin, Alpha Catenin 3
Reference Data

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