Desmin Mouse Monoclonal Antibody [Clone ID: RD301]

Specifications

Product Data
Clone Name	RD301
Applications	IF, IHC, WB
Recommend Dilution	Western blot. Immunohistochemistry on Frozen sections. Immunocytochemistry. Recommended Dilutions: 1/100-1/200 for Immunohistochemistry with Avidin-Biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 for Immunoblotting applications.
Reactivity	Canine, Chicken, Hamster, Human, Mouse, Porcine, Rabbit, Rat, Zebrafish
Host	Mouse
Clonality	Monoclonal
Immunogen	Cytoskeletal desmin extract of chicken gizzard
Specificity	RD301 reacts exclusively with Desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.
Formulation	PBS with 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles.
Database Link	UniProt ID P02542
Background	Desmin (53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.
Synonyms	DES
Reference Data

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