CEACAM5 Mouse Monoclonal Antibody [Clone ID: PARLAM4]

Specifications

Product Data
Clone Name	PARLAM4
Applications	FC, IF, IHC, WB
Recommend Dilution	Immunoblotting. Flow Cytometry. Immunocytochemistry on Methanol fixed cells. Immunohistochemistry on Frozen Sections (PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2). Immunohistochemistry on Paraffin Sections (Microwave retrieval method with citrate buffer pH 6.0 in an autoclave is required). Recommended Working Dilutions: 1/25–1/100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100–1/500 for immunoblotting application. Recommended Positive Control: Human Colon carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Isolated from Human colon carcinoma cells.
Specificity	Most polyclonal CEA antisera show cross-reactivity with related antigens such as biliary glycoprotein (BGP) and non-specific cross-reacting antigen 1/11 (NCA). PARLAM4 does not show cross reactivity, neither with BGP nor with NCA. In immunoblotting the antibody recognizes a single band of 180 kDa. This monoclonal antibody is reactive with human carcinoembryonic antigen (CEA), a tumour associated antigen with oncofetal characteristics. Although CEA can be found in tissues of non-neoplastic diseases and normal epithelia, it occurs also in a large variety of carcinomas. Therefore, immunohistochemical detection of CEA is frequently used for the histopathological diagnosis of Human tumours.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	carcinoembryonic antigen related cell adhesion molecule 5
Database Link	Entrez Gene 1048 Human UniProt ID P06731
Background	Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
Synonyms	CEA, Carcinoembryonic antigen
Reference Data

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