Collagen IV (COL4A1) Mouse Monoclonal Antibody [Clone ID: 1042]

Specifications

Product Data
Clone Name	1042
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (Proteolytic treatment required, Enzyme: Pepsin). Working Dilutions: 1/10-1/20 Incubation Time: 1 h at RT Recommended Positive Control: Skin, kidney.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human placental Collagen type IV
Specificity	Antibody 1042 is reactive with Human collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antibody is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. No crossreactivity with other Collagens.
Formulation	PBS State: Ascites State: Liquid Ascites Preservative: 0.01% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	collagen type IV alpha 1 chain
Database Link	Entrez Gene 1282 Human UniProt ID P02462
Background	Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
Synonyms	COL4A1
Reference Data
Protein Pathways	ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer

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