Desmoglein 2 (DSG2) Mouse Monoclonal Antibody [Clone ID: G129]

Specifications

Product Data
Clone Name	G129
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot. Immunohistochemistry on Frozen Sections: Ready-to-use (1 h at RT). Preincubation with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type, is recommended. Immunohistochemistry on Paraffin Sections: Ready-to-use (overnight at 2-8°C). Enhanced when using the microwave method. Recommended as Positive Control: CaCo-2, HaCaT.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant peptide (220 aa of 2nd and 3rd extracellular repeat domain).
Specificity	This antibody recognizes Desmoglein 2 present in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells Desmoglein 2 expression is generally very low. Reactivities on Cultured Cell Lines: Many, including CaCo-2, HaCaT, MCF-7. Negative Species: Mouse, Rat.
Formulation	State: Supernatant State: Liquid Cell Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	desmoglein 2
Database Link	Entrez Gene 1829 Human UniProt ID Q14126
Background	Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.
Synonyms	DSG2, CDHF5,HDGC
Note	Desmoglein 2 (1069 aa; calculated mol. wt. 116,760)
Reference Data

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