Desmoglein 1 (DSG1) Mouse Monoclonal Antibody [Clone ID: Dsg1-P23]

Specifications

Product Data
Clone Name	Dsg1-P23
Applications	IHC, WB
Recommend Dilution	Immunoblotting. Immunohistochemistry on Frozen Sections: Ready-to-use. Incubation Time: 1 h at RT. preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended (Ref.2). Immunohistochemistry on Paraffin Embedded Sections (after MICROWAVE treatment): Ready-to-use. Incubation Time: 1 h at RT. For better staining on paraffin sections preincubation with 0.001% trypsin and addition of 2% milk powder to the incubation media is recommended.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant Human polypeptide Desmoglein 1 (extracellular part EII, EIII and EIV).
Specificity	In immunoblots of cytoskeletal preparations from human skin the antibody reacts specifically with a 160 kD polypeptide typical for Desmoglein 1. In immunolocalization studies the mab shows typical staining of suprabasal layers of the epidermis and special regions of fetal and anagen hair follicles. The staining pattern observed corresponds to those obtained with pemphigus foliaceus patient sera. Cross-reaction with Desmogleins 2 and 3 was not detected.
Formulation	State: Supernatant State: Liquid Culture Supernatant Preservative: 0.09% Sodium Azide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	desmoglein 1
Database Link	Entrez Gene 1828 Human UniProt ID Q02413
Background	Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. Desmoglein 1 has been identified as the autoantigen of the autoimmune skin blistering disease pemphigus foliaceus. Desmoglein 1 is a 165kD glycoprotein that is a core component of the desmosome cell-cell junction between epithelial cells. Desmoglein 1 is synthesized in the suprabasal layer of the skin, and is vital to epidermal integrity.
Synonyms	Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, DSG1, CDHF4, DG1, DGI
Reference Data

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