Symplekin (SYMPK) Mouse Monoclonal Antibody [Clone ID: Sym-TJ-E150]

Specifications

Product Data
Clone Name	Sym-TJ-E150
Applications	IHC, WB
Recommend Dilution	Immunoblotting. Immunohistochemistry: For nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash.
Reactivity	Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human symplekin
Specificity	The mab reacts specifically with symplekin (a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelia!) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis; squamous cell carcinoma; nonepithelia such as fibroblasts, endothelia, lymphocytes).
Formulation	State: Supernatant State: Liquid culture supernatant
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Gene Name	symplekin
Database Link	Entrez Gene 8189 Human UniProt ID Q92797
Background	Symplekin, 126.5 kD polypeptide (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5
Synonyms	SPK
Reference Data

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