ADFP (PLIN2) (5-27) Mouse Monoclonal Antibody [Clone ID: AP125]

Specifications

Product Data
Clone Name	AP125
Applications	IF, IHC, WB
Recommend Dilution	Immunohistochemistry on Frozen Tissue: 1/10 - 1/100 in PBS, pH 7.4 (See Ohsaki et al. for staining protocols). Immunohistochemistry on Paraffin-Embedded Tissue: 1/10 - 1/100 (After microwave treatment). Incubate 1 h at RT or over night at 2-8°C. (See Straub et al. 2008 for staining protocols). Cytological Material. Immunoblotting (Western blot): 0.2 µg/ml using ECL has been reported for previous batch numbers.
Reactivity	Canine, Human, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Synthetic peptide corresponding to aa 5-27 from N-terminus of Human Adipophilin.
Specificity	Polypeptide Reacting: Adipophilin / ADRP, MW 48,100 (calculated from aa sequence data); apparent Mr 52,000 (after SDS-PAGE); pI 6.72. Tissue Immunolocalization: Adipophilin is positively detected in the glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, and in fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver; adipocytes are negative. Also positively stained are lipid-storing CD 68-positive macrophages. Tested Reactivity on Cultured Cell lines: Caco, PLC, HaCat, SV80, RD 125, Huvec (Human umbilical cord endothelia), RV, PC-12 (rat adrenal gland), MDCK. Negative with glioma. Negative Species: Bovine and Mouse.
Formulation	Final solution contains 0.09% Sodium Azide and 0.5% BSA in PBS buffer, pH 7.4 State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore with 1.0 ml distilled water
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	perilipin 2
Database Link	Entrez Gene 123 Human UniProt ID Q99541
Background	Adipophilin / ADRP / PLIN 2 is a ubiquitous component of lipid droplets. It has been found in milk fat globule membranes and on the surface of lipid droplets in various cultured cell lines (see e.g. Heid et al. 1998; for review see e.g. Targett-Adams et al.); inducible by etomoxir. Enhanced expression of Adipophilin / ADRP / PLIN2 is a useful marker for pathologies characterized by increased lipid droplet accumulation. Such diseases include atheroma, steatosis, obesity and certain cases of liposarcoma. It also seems to be a potent marker for atherosclerosis. ADRP can also be used to study virus entry via lipid droplets (see e.g. Hope et al., Samsa et al.).
Synonyms	Adipose differentiation-related protein, ADRP
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.