CDK4 Mouse Monoclonal Antibody [Clone ID: DCS-35]

Specifications

Product Data
Clone Name	DCS-35
Applications	IP, WB
Recommend Dilution	Western Blot. Immunoprecipitation: 1/3-1/10. Incubation Time: 1-4h at RT or overnight at 2-8°C.
Reactivity	Human, Mouse, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Human recombinant full-length cdk4 polypeptide
Specificity	This antibody reacts specifically with cdk4 protein present predominantly in G1 phase of cell cycle. It does not cross-react with cyclins. Especially suitable for Immunoprecipitation, mab DCS-35 precipitates the cdk4/cyclin complex (epitope localization: aa 1-20). Cellular Localization: Nuclear, Cytoplasmic. Mol. Wt. of Antigen: 34kDa
Formulation	PBS, pH 7.4 State: Purified State: Lyophilized purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Reconstitution Method	Restore in 1 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	cyclin-dependent kinase 4
Database Link	Entrez Gene 1019 Human UniProt ID P11802
Background	Cdk4 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. Cdk4 is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1/S phase, which is controlled by the regulatory subunits D type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). The mutations in this gene as well as its related proteins including D type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Two alternatively spliced variants, and multiple polyadenylation sites of this gene have been reported.
Synonyms	PSK-J3
Reference Data

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