Desmoplakin (DSP) Mouse Monoclonal Antibody [Clone ID: DP-2.17]

Specifications

Product Data
Clone Name	DP-2.17
Applications	IHC, WB
Recommend Dilution	Immunoblotting (Western blot). Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Sections (After Microwave treatment). Working Dilution: Dilute 1/10 with PBS (pH 7.4) immediately befor use for Immunohistochemistry. Incubation Time: 1h at RT.
Reactivity	Bovine, Chicken, Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Bovine Desmoplakin 1
Specificity	DP 2.17 shows distinct punctate membrane staining of epithelia. It reacts with the rod fragment of Desmoplakin 1 (Mr 250,000 polypeptide of desmosomal plaques). Tested Reactivities on Cultured Cell Lines: Several Human carcinoma cell lines: MCF-7, A-431, TR-146. Bovine cells: MDBK, BMGE.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide and 0.5% BSA State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml distilled water.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	desmoplakin
Database Link	Entrez Gene 306871 RatEntrez Gene 1832 Human UniProt ID P15924
Background	Desmosomes are intercellular junctions that form tight links between adjacent cells. Desmoplakin is an obligate component of functional desmosomes that attaches intermediate filaments to desmosomal plaques. It is involved in the organization of desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains. The N-terminus of desmoplakin is essential for localisation to the desmosome and interaction with plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds to intermediate filaments. The central region of desmoplakin comprises a coiled-coil rod domain that mediates homodimerisation. There are two isoforms of desmoplakin - desmoplakin I, which is an obligate component of all desmosomes, and desmoplakin II, which is predominantly expressed in tissues and cells of stratified origin. Mutations in the gene encoding desmoplakin result in a number of cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus.
Synonyms	DP, DSP, 250/210 kDa paraneoplastic pemphigus antigen
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Arrhythmogenic right ventricular cardiomyopathy (ARVC)

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