Desmoglein 2 (DSG2) Mouse Monoclonal Antibody [Clone ID: 10G11]

Specifications

Product Data
Clone Name	10G11
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western blot. Immunohistochemistry on Frozen Sections:  For better resolution preincubation (directly after fixation) with 0.05-0.2% Triton X-100 for 5-10 min, depending on tissue type, is recommended (See Ref.3). Immunohistochemistry on Paraffin Sections: Use microwave treatment (See also Ref.5). Incubation Time: 1 h at RT for Frozen tissue and overnight at 2-8°C for Paraffin Sections. Working Dilution : Dilute 1/10 with PBS, pH 7.4 for Immunohistochemical applications. Recommended Positive Control : CaCo-2, HaCaT.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant peptide (220aa of 2nd and 3rd extracellular repeat domain)
Specificity	The antibody recognizes Desmoglein 2 present in low concentrations in all epithelia and in tumors derived therefrom as well as in heart muscle and dendritic reticulum cells of lymphatic follicles. In basal epithelial cells desmoglein 2 expression is generally very low. Polypeptide Reacting: Desmoglein 2 (1069aa; calculated Mol. Wt. 116,760). Reactivity on Cultured Cell Lines: Many, including CaCo-2, HaCaT, MCF-7.
Formulation	Final solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in 1 ml distilled water
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	desmoglein 2
Database Link	Entrez Gene 1829 Human UniProt ID Q14126
Background	Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmoglein 2 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18. This second family member is expressed in colon, colon carcinoma, and other simple and stratified epithelial-derived cell lines.
Synonyms	DSG2, CDHF5,HDGC
Reference Data

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