Histone H1 Mouse Monoclonal Antibody [Clone ID: AE-4]

Specifications

Product Data
Clone Name	AE-4
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA. Western blotting. Immunoprecipitation. Immunofluorescence. Immunhistology on Frozen and Paraffin Sections: Acetone or PFA fixation is recommended for Frozen sections. Positive Control Tissue: Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	A BALB/c mouse was immunized with Human leukemia biopsy cells. The isolated splenocytes were fused with mouse myeloma cells.
Specificity	AE-4 recognizes Histone H1 in the nuclei of animal and plant cells. It produces a speckled nuclear staining pattern in normal and malignant cells. Tonsil is the recommended positive control tissue.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.02% Sodium Azide
Concentration	lot specific
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Background	The histone proteins serve as a structural scaffold for the organisation of nuclear DNA into chromatin. In eucariotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that asseble to form an octamer round which the DNA is wrapped in a sequence dependant manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30 nm chromatin fiber.
Synonyms	H1 Histone Family, H1F4, H1F5, Histone H1a, Histone H1b
Reference Data

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