Cd4 Mouse Monoclonal Antibody [Clone ID: Rib 5/2]

Specifications

Product Data
Clone Name	Rib 5/2
Applications	FC, FN, IHC
Recommend Dilution	Suitable for Immunohistochemistry (IHC); has been described to work in FACS (see Table 1). Recommended Dilutions for IHC: Frozen sections: 1.5 μg/ml (1/200) Paraffin sections: does not react on routinely processed paraffin sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Lymphoblasts.
Specificity	Recognizes a subpopulation of rat T-cells (T-lymphocytes and thymocytes, monocytes, macrophages). Rib 5/2 detects an epitope of the CD4 53kD glycoprotein on the cell surface. It is used for the identifcation of CD4 bearing cells, mainly T-helper / inducer lymphocytes. Rib 5/2 stains T-cell areas in the spleen and lymphnodes well. In the thymus Rib 5/2 detects cortical and medullary thymocytes. It recognises tissue macrophages and Th-cells according to the CD 4 distribution. It can also be applied for in vivo blocking of CD4 mediated immunological reactions.
Formulation	PBS, pH 7.2 containing 0.01% Thiomerosal as preservative and 10 mg/ml BSA as stabilizer. State: Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.3 mg/ml
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	Cd4 molecule
Database Link	Entrez Gene 24932 Rat UniProt ID P05540
Background	CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.
Synonyms	T-cell surface antigen T4/Leu-3
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum
Reference Data

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