Tfrc Rat Monoclonal Antibody [Clone ID: ER-MP21]

CAT#: BM4087B

Tfrc rat monoclonal antibody, clone ER-MP21, Biotin

Conjugation: Unconjugated Biotin



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CNY 8,588.00


货期*
5周

规格
    • 200 ug

Product images

Specifications

Product Data
Clone Name ER-MP21
Applications FC, IHC
Recommend Dilution Flow Cytometry (5 µg): Use 5 µl of the suggested dilution to label 106 cells.
Immunohistochemistry on Frozen Sections: 2 µg/ml (1/200).
Does not react on routinely processed Paraffin Sections.
Suggested positive control: Mouse spleen.
Reactivity Mouse
Host Rat
Clonality Monoclonal
Immunogen Macrophages precursor cells.
Specificity ER-MP21 stains proliferating cells of all types as well as cells taking up iron for special needs, such as late erythroid precursors and some mature macrophages. The antibody inhibits the iron uptake and the proliferation of macrophage precursors from bone marrow and early macrophage precursor cell lines.
ER-MP21 recognizes the transferrin receptor but does not compete with transferrin binding.
The antigen is a 200 kD protein (non-reducing conditions) consisting of two identical chains. The recognized epitope of the receptor is not located at the transferrin binding site.
Antigen Distribution:
Macrophage progeny at day 7 in bone marrow cultures, treated in parallel on various days by irradiation (1500 rad 60Co γ-radiation) or addition of ER-MP21 (30 µg/ml final concentration).
Cell numbers were quantified using the MTT assay and expressed relative to untreated controls. Relative growth inhibition calculated from data represented in left figure. For mAbor radiation treated cultures, the number of cell cycles needed to reach the cell number in untreated controls was calculated. Values obtained for irradiated cultures were taken as 100% inhibition controls and values for ER-MP21 - treated cultures were expressed relative to these (See Figure. 1).
Formulation Stock solution contains PBS, pH 7.2 with 0.09% Sodium Azide as preservative and 10 mg/ml BSA as stabilizer
Label: Biotin
State: Lyophilized purified Ig fraction
Reconstitution Method Restore with 0.5 ml distilled water.
Concentration 0.4 mg/ml (after reconstitution)
Purification Affinity Chromatography
Conjugation Biotin
Storage Condition

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Gene Name transferrin receptor
Background The transferrin receptor has been structurally characterized as a sulfide bound dimer of identical glycoprotein subunits of 95 kDa. The transferrin receptor is not present on resting blood lymphocytes. On PBL, the receptor appears after activation. The expression of transferrin receptor is coordinately regulated with cell growth. Present on T and B cell lines. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.
Synonyms TfR1, p90, Transferrin receptor protein 1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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