Collagen IV (COL4A1) Mouse Monoclonal Antibody [Clone ID: CIV22]

Specifications

Product Data
Clone Name	CIV22
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections: 0.125 µg/ml (1/1600). Immunohistochemistry on Paraffin Sections: 0.5 µg/ml (1/400). Proteinase K pretreatment for antigen retrieval is recommended. Also suitable for ELISA. Recommended Positive Control: Human tonsil. The antigen is stable to formalin fixation, however.
Reactivity	Bovine, Human, Porcine
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified pepsin fragments of Human type IV Collagen from Human kidneys.
Specificity	This Monoclonal antibody Clone CIV22 stains basement membranes in a variety of tissues and organs, including kidney, skin, striated and smooth muscle, spleen, lymph node, lung, placenta, and tendon. It recognizes native, but not denatured Collagen type IV but nevertheless reacts clearly with formalin-fixed paraffin-embedded sections. CIV22 is a useful marker for monitoring destruction of basal membranes as observed in malignant neoplastic diseases. This antibody recognizes a conformational epitope on a helical part of native Collagen IV and loses its reactivity on denaturing the Collagen IV protein. Antigen Distribution on Tissue Sections: Basically all basement membranes except corneal epithelium.
Formulation	PBS, pH 7.2 State: Purified State: Lyophilized purified IgG fraction. Production: This antibody was produced Serum-free, without Fetal Calf Serum. Stabilizer: 5 mg/ml BSA Preservative: 0.05% (v/v) Kathon CG
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.2 mg/ml (after reconstitution)
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	collagen type IV alpha 1 chain
Database Link	Entrez Gene 1282 Human UniProt ID P02462
Background	Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
Synonyms	COL4A1
Note	Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum. Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.