CEACAM5 Mouse Monoclonal Antibody [Clone ID: C19]

Specifications

Product Data
Clone Name	C19
Applications	IHC
Recommend Dilution	Suitable for Immunohistochemistry on paraffin embedded sections (0.1 µg/ml, 1/4000). Microwave pretreatment for antigen retrieval is recommended. Suggested positive control: Human colon carcinoma.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Specificity	C19 is useful to detect CEA in malignant adenocarcinoma, notably in the gastro-intestinal tract. Clinical relevance is also found in breast-, lung-, prostatic-,ovarian- and other carcinomas of endodermal origin (stomach, liver, pancreas). Contrary to some other antibodies, C19 stains granulocytes in blood smears and tonsils, as well as epithelial cells.
Formulation	PBS, pH 7.2 with 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative. State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions Avoid repeated freezing and thawing.
Gene Name	carcinoembryonic antigen related cell adhesion molecule 5
Database Link	Entrez Gene 1048 Human UniProt ID P06731
Background	CEA is a glycoprotein of 180kDa m.w. It is a normal constituent of the glycocalix of embryonic endodermal epithelium and is produced mainly in the mucosa of the normal colon.
Synonyms	CEA, Carcinoembryonic antigen
Note	Protocol: Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature 1. Deparaffinize and rehydrate tissue section 2. Place slide in a cuvette with 250ml 0.01M citrate buffer, pH 6.0 3. Heat slide in a microwave oven for 2 x 7min. at 700 Watt 4. Leave slide in the buffer for 20min for cooling 5. Wash in distilled water 6. Block endogenous peroxidase 7. Wash in PBS 8. Block with 10% normal goat serum in PBS for 30min. in a humid chamber 9. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 10. Wash in PBS 11. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM (H+L) minimal-cross reaction to human) for 1h in a humid chamber 12. Wash in PBS 13. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 14. Wash in PBS 15. Counterstain with Mayer's hemalum.
Reference Data

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