CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-25]

Specifications

Product Data
Clone Name	MEM-25
Applications	FC, FN, IP
Recommend Dilution	Flow Cytometry. Immunoprecipitation (excellent for immunoaffinity purification of LFA-1 complex)). Functional applications (MAb MEM-25 partially blocks binding of LFA-1 complex to ICAM-1).
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Leucocytes from a pacient suffering from a LGL-type leukaemia
Specificity	The antibody clone MEM-25 reacts with the alpha-subunit (CD11a) of human LFA-1, a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Formulation	Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 State: Azide Free State: Liquid Ig fraction (> 95% pure)
Concentration	lot specific
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha L
Database Link	Entrez Gene 3683 Human UniProt ID P20701
Background	CD11a together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Synonyms	Integrin alpha-L, LFA1, LFA-1
Reference Data

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