CD33 Mouse Monoclonal Antibody [Clone ID: MY9]

Specifications

Product Data
Clone Name	MY9
Applications	FC, IHC, IP
Recommend Dilution	Immunohistochemistry on Frozen Sections: 4 µg/ml (1/100). FACS: 10 µl stock solution to label 106 cells. Immunoprecipitation. Suggested Positive Control: Human tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	The antigen is CD33
Specificity	The CD33 antibody is valuable for distinguishing acute myeloid from lymphoblastic leukaemias. CD33 is expressed on monocytes in peripheral blood. In the bone marrow, CD33 antigen is found on granulocyte and macrophage precursors, and is absent from pluripotent stem cells.
Formulation	PBS, pH 7.2 State: Purified State: Lyophilized purified Ig fraction Stabilizer: 5 mg/ml BSA Preservative: 0.05% (v/v) Kathon CG
Reconstitution Method	Restore with 0.5 ml distilled water.
Concentration	0.4 mg/ml (after reconstitution)
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	CD33 molecule
Database Link	Entrez Gene 945 Human UniProt ID P20138
Background	CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias. Identification of human Monocytes (bright) and Granulocytes (dim) expressing the 67kDa M.W. surface antigen. CD33 is a 67kDa single chain transmembrane glycoprotein that belongs to the sialoadhesin family.
Synonyms	Siglec-3, gp67
Reference Data

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