CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: X-5]

CAT#: BM4032B

CD11b (ITGAM) mouse monoclonal antibody, clone X-5, Biotin

Conjugation: Unconjugated Biotin



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CNY 7,875.00


货期*
5周

规格
    • 750 ug

Product images

Specifications

Product Data
Clone Name X-5
Applications IHC
Recommend Dilution Suitable for Immunohistochemistry on frozen sections: 7.5 - 30 µg/ml (1/50-1/200).
Suggested positive control: Human tonsil
Antigen Distribution on Isolated cells and Tissue sections: Weakly positive on granulocytes. Positive on peritoneal macrophages. Negative on lymphocytes.
Cell Lines: HL-60: positive on approx. 50% following 1.25% DMSO treatment increased to 100%.
In lymph nodes X-5 is positive on monocytes, macrophages and single cells both intrafollicularly and in surrounding blood vessels. Positive staining has been observed with the basal membrane of blood vessels and on sinus walls. Marginal zone macrophages, medulla and red pulp stain positive in the spleen as do cells in the T-cell region. In the thymus, medulla and trabecular macrophages are positive.
Reactivity Human
Host Mouse
Clonality Monoclonal
Immunogen Human peripheral blood monocytes
Specificity This antibody reacts with granulocytes, monocytes, NK- cells.
X-5 recognizes an antigen of 155/95kD determined by Immunoprecipitation.
Formulation PBS, pH 7.2 with 0.01% Thimerosal as preservative and 10 mg/ml BSA as stabilizer.
Label: Biotin
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore with 0.5 ml distilled water.
Concentration 1.5 mg/ml
Purification Affinity chromatography.
Conjugation Biotin
Storage Condition Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Do not freeze working dilutions
Avoid repeated freezing and thawing.
Gene Name integrin subunit alpha M
Background Antibody X-5 recognizes a non-covalently bound alpha heterodimeric glycoprotein. It belongs to the ß-2 integrin supergene family of adhesion. The antigen is a receptor for C3bi, fibrinogen, coagulation factor X. Together with CD18, CD11b forms the MAC-1 molecular complex involved in the adhesion to endothelial cells, and the subsequent extravasation of neutrophils and monocytes.
Synonyms ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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