Collagen I (COL1A1) Mouse Monoclonal Antibody [Clone ID: NFI/20]

Specifications

Product Data
Clone Name	NFI/20
Applications	ELISA, IHC
Recommend Dilution	ELISA: 1/500-1/2000. Immunohistochemistry on Frozen Sections: 1/10-1/40.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human Collagen type I from placenta.
Specificity	This antibody clone NFI/20 is specific for Human Collagen I and does not recognize Collagen types II, III and IV.
Formulation	Buffer: 0.1M Sodium Chloride, 0.01M Sodium Phosphate, 0.01M Sodium Borate, with 1% Mannitol and 1% Dextran. State: Purified State: Lyophilized Ig fraction.
Reconstitution Method	Restore with 1 ml of sterile distilled water.
Purification	Ammonium Sulphate Precipitation and DEAE-Cellulose Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	collagen type I alpha 1
Database Link	Entrez Gene 1277 Human UniProt ID P02452
Background	Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon.  Mutations in the encoding gene are associated with osteogenesis imperfecta (Peng et al. 2012), Ehlers Danlos syndrome (Nuytinck et al. 2000) and idiopathic juvenile osteoporosis (Pocock et al. 1995). Defective processing of collagen I by lysyl hydroxylase has also been implicated in Ehlers Danlos syndrome (Ihme et al. 1984).
Synonyms	COL1A1, COL1A2, Alpha-1 type I collagen, Alpha-2 type I collagen
Reference Data
Protein Families	Druggable Genome
Protein Pathways	ECM-receptor interaction, Focal adhesion

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