TIA1 Mouse Monoclonal Antibody [Clone ID: 2G9A10F5]

Specifications

Product Data
Clone Name	2G9A10F5
Applications	IHC
Recommend Dilution	Immunohistochemistry on Frozen Sections. Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/100 Pretreatment of deparaffinized tissue with heat-induced epitope retrieval is recommended. Use Polymer anti Mouse/Rabbit IgG as a detection system. Positive Control: Tonsil.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human bone marrow malignant cells from a non-B, non-T acute leukemia.
Specificity	Within hematopoietic cell lines, the 'TIA-1' monoclonal antibody (2G9) reacts with about 90% of CD16+, 50-60% of CD8+, and less than 10% of CD4+ normal peripheral blood lymphocytes. (5) It reacts with almost all monocytes and granulocytes. (3) This antibody also reacts with CD4+ activated T-cell clones, activated NK cell clones, and Con A-activated thymocytes, but not with B lymphocytes or B-cell lines. (1,5) The 2G9A10F5 monoclonal antibody was evaluated during the 5th International Workshop on Human Leucocyte Differentiation Antigens, in Boston, USA, in the section of monoclonal antibodies reactive with intracellular antigens. (6) Cellular Localization: Cytoplasmic.
Formulation	State: Purified Buffer: Tris‐HCI containing stabilizing protein and <0.1% sodium azide.
Purification	Protein G Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Gene Name	TIA1 cytotoxic granule-associated RNA binding protein
Database Link	Entrez Gene 5395 HumanEntrez Gene 7072 Human UniProt ID P31483
Background	TIA1 is a member of an RNA binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 17-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing.
Synonyms	Nucleolysin TIA-1, p40-TIA-1, p15-TIA-1
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Mismatch repair

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