CD44 Mouse Monoclonal Antibody [Clone ID: 156-3C11]

Specifications

Product Data
Clone Name	156-3C11
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted CD44 antibody to label 1x10e6 cells in 100 µl. Immunoprecipitation. Western Blot: Detects a band of approximately 80kDa in HeLa cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: 1/100. This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommneded Positive Control: Human tonsil.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Stimulated Human leucocytes.
Specificity	This antibody recognises CD44, a 90kD type I transmembrane protein which is otherwise known as HCAM or CD44s. Various isoforms of CD44 exist. The 90kD standard form, recognised by this antibody, is expressed on leucocytes, erythrocytes, white matter of the brain and some epithelial cells of the breast and small intestine. Antibodies produced by clone 156-3C11 recognise epitope 3, which is a protease resistant epitope on the CD44 molecule.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD44 molecule (Indian blood group)
Database Link	Entrez Gene 960 Human UniProt ID P16070
Background	CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential.
Synonyms	LHR, MDU2, MDU3, MIC4, CDw44, Epican, ECMR-III, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, PGP-1
Reference Data

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