EPCAM Mouse Monoclonal Antibody [Clone ID: HEA125]

Specifications

Product Data
Clone Name	HEA125
Applications	FC, IF, IHC
Recommend Dilution	Flow Cytometry: Use 5 µl of neat antibody to label 106 cells or 100 µl cell suspension. Immunofluorescence. Immunohistochemistry on Frozen Sections and Paraffin Sections: 1/20. This product requires protein digestion pre-treatment of paraffin sections e.g. 0.1% pronase, 10 minutes. Recommended Positive Control: Human colon and appendix.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	HT29 carcinoma cell line. Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.
Specificity	This antibody recognizes the 34kDa cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM).
Formulation	PBS, pH 7.3 State: Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	epithelial cell adhesion molecule
Database Link	Entrez Gene 4072 Human UniProt ID P16422
Background	Epithelial Cell Adhesion Molecule (EpCAM) is a 40 kDa cell surface antigen. This antigen has been identified independently by a number of groups, and has been known by a variety of names. Several monoclonal antibodies have been raised against EpCAM, many of which have been described as tumour specific molecules on carcinomas. EpCAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. EpCAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.
Synonyms	Ep-CAM, Epithelial cell adhesion molecule, GA733-2, EGP314, KSA, TROP1, Trop-1, M1S2, M4S1, MIC18
Reference Data

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