26S Proteasome (p32 subunit) Mouse Monoclonal Antibody [Clone ID: p32(26S-161)]

Specifications

Product Data
Clone Name	p32(26S-161)
Applications	IF, IHC, WB
Recommend Dilution	Western Blot: 1/1000 for Immunoblotting when using the ECL method. Immunofluorescence Microscopy: Ready-to-use. Incubation Time: 20-30 min at RT (longer incubation may result in loss of soluble antigen). P32 (26S-161) is not suitable for Immunoprecipitation. This Clone antibody has been reported to work in Immunohistochemistry on Frozen Sections (See Reference 4. for more details).
Reactivity	Human, Rat, Xenopus
Host	Mouse
Clonality	Monoclonal
Immunogen	26S complexes of Xenopus laevis oocytes.
Specificity	Recognizes the 20S subcomplex within the 26S heterooligomeric protein complex and the free cytosolic form of 20S cylinder particles. The epitope is resistant to formaldehyde fixation (up to 4%). Antigen Recognized in Species and Cultured Cell Lines (tested so far): Xenopus laevis, Human; XLKE-A6 (Xenopus) PLC, MCF-7, A431, CaCo (Human); 3T3 (Mouse); RV (Rat); Ptk2 (Rat kangaroo).
Formulation	State: Supernatant State: Liquid Tissue Culture Supernatant containing 0.09% Sodium Azide as preservative
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C.
Background	The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.
Synonyms	Proteasome26S
Reference Data

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