Complement C3 (C3) Mouse Monoclonal Antibody [Clone ID: H11]

Specifications

Product Data
Clone Name	H11
Applications	IHC, WB
Recommend Dilution	Western blot. Immunohistochemistry on frozen sections: 1/10. Incubation time: 1h at RT. Successful use on paraffin embedded tissue was reported by some researchers. As this detection method has not been verified by Acris Antibodies, the application was deleted from the database. This does not necessarily exclude the use in such procedure.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human complement component C3
Specificity	Detects C3b deposits in tissue, on cells, on microorganisms and in immune complexes. Does not react with C3a or C3d. Inhibits hemolytic function of C3. It should be used with positive control anti C3b-α (clone H206, cat.no. BM5029), and negative control anti C3a (clone H13, cat.no. BM2131). Activated, deposited C3b bears 3b-α and -ß, but no longer C3a. Ubiquitous or nonspecifically absorbed C3 is still C3a positive with clone H13.
Formulation	PBS, pH 7.4, containing 0.09% sodium azide, 0.5% BSA State: Purified State: Lyophilized purified Ig fraction
Reconstitution Method	Restore with 1 ml dist. water.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	complement component 3
Database Link	Entrez Gene 718 Human UniProt ID P01024
Background	The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.
Synonyms	CPAMD1, Complement component 3
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Complement and coagulation cascades, Systemic lupus erythematosus

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