EGFL7 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, IP, WB
Recommend Dilution	Western blot: 2-5 µg/ml. Immunohistochemistry on Frozen sections. Immnunocytochemistry: 10 µg/ml. Immunoprecipitation: 1 µg/ml.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>95%) recombinant Human EGFL7 (Pro187-Ser273) C-terminus derived from E. coli.
Specificity	This antibody recognizes Human VE-Statin / EGFL7 at C-term. Other species not tested.
Formulation	PBS State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in sterile water/PBS to a concentration of 0.1-1.0 mg/ml.
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	EGF like domain multiple 7
Database Link	Entrez Gene 51162 Human UniProt ID Q9UHF1
Background	EGFL7 (epidermal growth factor-like domain 7) is a secreted angiogenic factor that is highly conserved in vertebrates. Most secreted angiogenic signaling molecules, including VEGF and FGF-2, are mainly expressed by non-endothelial cell types such as fibroblasts. In contrast, EGFL7 is unique because it is almost exclusively expressed by and acts on endothelial cells. EGFL7 expression is highest when the endothelium is in an active, proliferating state. This factor acts as a chemo-attractant for endothelial cells and binds to components of the extracellular matrix. In vivo, EGFL7 is important for regulating tubulogenesis in Zebrafish and for controlling vascular patterning and integrity in mice. Its function in blood vessel development is mediated, at least in part, through modulation of Notch signaling. There is evidence that support a role for EGFL7 in developmental and postnatal angiogenesis as well as a potential role for EGFL7 in vascular repair. EGFL7, also known as VE-Statin (Vascular endothelial Statin), is a endothelial-specific protein that is a marker for progenitor, embryonic and adult endothelial cells. Mature soluble EGFL7/VE-Statin is a 41 kDa protein containing an N-terminal cysteine rich EMI domain, followed by two EGF-like domains and a coiled-coil region. EGFL7/VE-Statin inhibits PDGF-BB-induced smooth muscle cell migration. The amino acid sequence of human EGFL7/VE-Statin is 80% identical to that of mouse EGFL7/VE-Statin.
Synonyms	EGF-like protein 7, EGF like protein 7, VE-statin, NOTCH4-like protein, Zneu1, MEGF7, UNQ187, PRO1449
Reference Data

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