Egfl7 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IP, WB
Recommend Dilution	Western blot: 1-5 µg/ml. Immnunocytochemistry. Immunoprecipitation.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure recombinant mouse EGFL7 (Glu181-Leu275; C-terminus) derived from E. coli
Specificity	This antibody recognizes VE-Statin/EGFL7.
Formulation	PBS State: Purified State: Lyophilized purified IgG fraction
Reconstitution Method	Restore in sterile water/PBS to a concentration of 0.1-1.0 mg/ml.
Purification	Protein A chromatography
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	EGF-like domain 7
Database Link	Entrez Gene 353156 Mouse UniProt ID Q9QXT5
Background	EGFL7 (EGF-like domain-containing protein 7) regulates vascular tubulogenesis in vivo. During sprouting angiogenesis, groups of endothelial cells (ECs) migrate together in units called sprouts. EGFL7 regulates the proper spatial organization of ECs within each sprout and influences their collective movement. It inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cells adhesion to the substrate in vitro. EGFL7 is an ∼ 30 kDa secreted protein that contain an Emilin-like (EMI) domain (a multimerization motif), and 2 epidermal growth factor (EGF) domains, one of which binds calcium. Based on these domains, it has been hypothesized that EGFL7 may self-assemble like extracellular matrix (ECM) proteins and, thus, could incorporate into ECM. EGFL7 has been reported to stimulate cell adhesion as well as motility in a manner similar to ECM proteins. EGFL7 has been shown to be primarily expressed by developing ECs but also by primordial germ cells and some central nervous system neurons. Interestingly, EGFL7 expression markedly decreases in ECs in postnatal life, but can be strongly up-regulated after various tissue injuries that lead to increased angiogenic responses.
Synonyms	EGF-like protein 7, EGF like protein 7, VE-statin, NOTCH4-like protein, Zneu1, MEGF7, UNQ187, PRO1449
Reference Data

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