PRKACA pThr197 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western blot: 1:500~1:1000. Immunohistochemistry on paraffin sections: 1:50~1:100. Immunofluorescence: 1:100~1:200.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide sequence around phosphorylation site of threonine 197(R-T-W(p)-T-L) derived from Human PKA α/β CAT (KLH-conjugated)
Specificity	The antibody detects endogenous levels of PKA α/β CAT only when phosphorylated at threonine 197.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Concentration	lot specific
Purification	Affinity chromatography using epitope-specific peptide
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Predicted Protein Size	40 kDa
Gene Name	protein kinase cAMP-activated catalytic subunit alpha
Database Link	Entrez Gene 5566 Human UniProt ID P17612
Background	Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
Synonyms	PKA C-alpha / PKA C-alpha
Reference Data

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