BubR1 (BUB1B) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot: 1/200-1/2000. Immunohistochemistry: 1/50-1/500.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from N-terminal domain of Human BUB1B
Specificity	Reacts with Human 115 kDa protein. Reacts with cleaved protein and cross reacts with Mouse BUB1 protein.
Formulation	State: Serum State: Lyophilized powder Preservative: None
Reconstitution Method	Restore in distilled water.
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store the antibody at -20°C. Store reconstituted antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	BUB1 mitotic checkpoint serine/threonine kinase B
Database Link	Entrez Gene 701 Human UniProt ID O60566
Background	Metaphase checkpoint controls sense abnormalities in chromosome alignment during mitosis and prevent progression to anaphase until proper alignment has been attained. A number of proteins, including Mitotic arrest deficiency protein 2 (MAD2), Budding uninhibited benzimidazole 1 (BUB1) and Budding uninhibited benzimidazole receptor 1 (BUBR1), have been implicated in the metaphase checkpoint control in mammalian cells. BUB1 and BUBR1 both localize to kinetochores during mitosis, suggesting that they play a role in delaying anaphase until all chromosomes achieve correct, bipolar attachment to the spindle. BUB1 and BUBR1 respond differently to spindle dynamics; they are part of a common complex during mitosis and BUB1 and BUBR1 may integrate different 'spindle assembly signals' into a single signal which can then be interpreted by downstream cell cycle regulators.
Synonyms	Protein SSK1, BUBR1, MAD3L, BUB1 beta
Reference Data

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