DOCK5 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	ELISA. Western Blot: 1/200-1/2000.  Immunohistochemistry: 1/50-1/500.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide derived from C-terminal domain of Dock5 protein
Specificity	This antibody reacts specifically with 23 and 215 kDa Mouse Dock5 proteins.   Cross reacts with 215 kDa Human protein.
Formulation	State: Serum State: Lyophilized serum Preservative: None
Reconstitution Method	Restore in distilled Water
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	dedicator of cytokinesis 5
Database Link	Entrez Gene 68813 Mouse UniProt ID B2RY04
Background	DOCK 5 (dedicator of cytokinesis protein 5) is a 1,870 amino acid protein belonging to the DOCK family of cytokinesis-regulating proteins. This cytoplasmic peripheral membrane protein activates Rac 1 and Rac 2 small GTPases, while presumably acting as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK 5 contains one DHR-1 (CZH-1) domain, one DHR-2 (CZH-2) domain and one SH3 domain. The DHR-2 domain is a putative GEF activity mediator. In mice, spontaneous mutation of the gene encoding DOCK 5 leads to deletion of the DHR-1 domain, which functions to bind phospholipids and assists in protein-protein interactions, resulting in rupture of lens cataract (RLC). Due to siRNA knockdown studies, it is suspected that DOCK 5 may also be an important mediator of CrkII/CrkL regulation of Caco-2 migration and spreading on COL4. There are two isoforms of DOCK 5 that exist as a result of alternative splicing events.
Synonyms	DKFZp451J181; DKFZp779M164; DKFZp781J211; FLJ21034
Reference Data

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