CDT2 (DTL) (Center) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, WB
Recommend Dilution	ELISA: 1/1000. Western Blot: 1/100-1/500.  Flow Cytometry: 1/10-1/50.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of Human DTL.
Specificity	This antibody recognizes Human and Mouse CDT2 / DTL (Center).
Formulation	PBS with 0.09% (W/V) Sodium Azide as preservative State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	denticleless E3 ubiquitin protein ligase homolog
Database Link	Entrez Gene 76843 MouseEntrez Gene 51514 Human UniProt ID Q9NZJ0
Background	Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
Synonyms	Denticleless protein homolog, CDW1, DCAF2, L2DTL, RAMP
Note	Molecular Weight: 79468 Da
Reference Data
Protein Families	Druggable Genome

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