RPL22 (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	ELISA: 1/16000 (Detection Limit).  Western blot: Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). This batch is not expected to cross-react with Mouse and Rat in Western blot. Immunohistochemistry on Paraffin Sections: 3-6 µg/ml. In paraffin embedded Human Brain (Cortex) shows cytoplasm staining in neuronal bodies. 3.75 µg/ml. In paraffin embedded Human Thyroid, Uterus and Testis.
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide (C-SKESYELRYFQINQ) from C-terminus of Human RPL22
Specificity	This antibody recognizes the C-term of RPL22.
Formulation	Tris saline buffer, pH~7.3 State: Aff - Purified State: Liquid purfied IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ribosomal protein L22
Database Link	Entrez Gene 6146 Human UniProt ID P35268
Synonyms	EBER-associated protein, EAP, HBp15
Reference Data

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