ZIC3 (178-189) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/64000 (Detection Limit). Western blot: Preliminary experiments gave an approx 70kDa band in Human, Mouse and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.6kDa according to NP_003404.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Paraffin Sections: 5-10 µg/ml. In paraffin embedded Human Prostate shows nuclear staining in select secretory cells.
Reactivity	Bovine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_003404.1.
Specificity	This antibody detects ZIC3 (178-189) and is expected NOT to cross-react with other ZIC proteins.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	Zic family member 3
Database Link	Entrez Gene 7547 Human UniProt ID O60481
Synonyms	HTX; HTX1; ZNF203
Reference Data

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