CFC1 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western Blot: Preliminary experiments gave an approx 55kDa band in Human Breast, Placenta and Testis lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 24.6kDa according to NP_115934.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry on Paraffin Sections: 1-2 µg/ml. In paraffin embedded Mouse Embryonic Liver shows staining of the hepatocyte cell membranes.
Reactivity	Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_115934.1.
Specificity	This antibody recognizes CFC1 / Cripto-1.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Slphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cripto, FRL-1, cryptic family 1
Database Link	Entrez Gene 55997 Human UniProt ID P0CG37
Synonyms	CFC1
Reference Data

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