Actin Chicken Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Quality Control: This anti-Actin preparation was validated by Western blotting on nitrocellulose membranes (at a concentration of 1 µg/ml) using HRP-Conjugated Goat anti-Chicken IgY (1/5000 dilution, Cat.-No AP31795HR-N) as the secondary reagent. Recommended Dilutions: 1/1000-1/2000 for Western blot analysis of Nitrocellulose membranes. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary. Do Not use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.
Reactivity	Rabbit
Host	Chicken
Clonality	Polyclonal
Immunogen	Chickens were immunized with actin purified from Rabbit muscle. After epeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 µg/ml of affinity purified anti-Actin IgY, and then filter-sterilized.
Formulation	Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) State: Aff - Purified State: Liquid purified IgY fraction Preservative: 0.02% (w/v) Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at -20°C This product is photosensitive and should be protected from light.
Reference Data

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