PRAK (MAPKAPK5) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: 0.5-1 μg/ml. In transfected HEK293 transiently expressing either Mouse MK5 fused to GFP or the similar Mouse MK2 fused to GFP; only the expected band was observed in the MK5 lane after AP32808PU-N labeling. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced wiith Mk5/Erk3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 µg AP32808PU-N or AP32809PU-N. Western blots of the Immunoprecipitation were labelled with Mouse anti-MK5 (SC) or with Rabbit anti-ERK3 serum (CST). An approx 55kDa of MK5 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression constructas described in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunohistochemistry on Paraffin Sections: 4-8 µg/ml. In paraffin embedded Human Kidney shows brushborder staining in the PCT.
Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_003659.2; NP_620777.1.
Specificity	Recognizes MK5 / MAPKAPK5.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	mitogen-activated protein kinase-activated protein kinase 5
Database Link	Entrez Gene 8550 Human UniProt ID Q8IW41
Synonyms	MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK5, PRAK
Reference Data

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