APPL (APPL1) (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Peptide ELISA: 1/2000 (Detection Limit). Western blot: 0.3-1.0 μg/ml. Detects a band of Approx 90kDa in Human Heart lysates (calculated MW of 79.7kDa according to NP_036228.1). A customer has reported that this antibody also works well in Mouse (hepatocytes, C2C12 myoblasts and Mouse embryonic fibroblast cells).
Reactivity	Human, Mouse
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the C-Terminus of the protein sequence according to NP_036228.1.
Specificity	Recognizes Human APPL / DIP13 alpha (C-term). Other species not tested.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
Database Link	Entrez Gene 26060 Human UniProt ID Q9UKG1
Background	The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus.
Synonyms	Dip13-alpha, KIAA1428
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.