LC3C (MAP1LC3C) (N-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	IHC, WB
Recommend Dilution	ELISA: 1/1,000. Western blot: 1/1,000. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of Human LC3 (APG8c).
Specificity	This antibody recognizes Human, Mouse and Rat LC3C (APG8C) (N-term). Other species not tested.
Formulation	PBS containing 0.09% (W/V) Sodium Azide as preservative State: Purified State: Liquid puirified Ig fraction
Concentration	lot specific
Purification	Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	microtubule associated protein 1 light chain 3 gamma
Database Link	Entrez Gene 440738 Human UniProt ID Q9BXW4
Background	Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3c is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
Synonyms	Microtubule-associated protein 1 LC3 gamma, MAP1A/1B light chain 3 C, MAP1A/MAP1B LC3 C, MAP1 light chain 3-like protein 3
Reference Data

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