Prealbumin / Transthyretin Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	Peptide ELISA: 1/1000 (Detection limit).  Enzyme Immunoassay: 5-10 µg/ml. Sandwich-type ELISA with increasing amount of recombinant TTR captured by a Rabbit antibody. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Positive Control: Human Pancreas.
Reactivity	Canine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Synthetic peptide (C-YKVEIDTKSYWK) from the internal region of the protein sequence according to Human Prealbumin (NP_000362.1).
Specificity	This antibody recognizes Prealbumin / Transthyretin.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Prealbumin is a serum protein usually occurring at 200-400mg/L serum. Prealbumin migrates faster than albumin in acidic starch gels and include alpha 1 antitrypsin, thyroxine binding prealbumin, and orosomucoid, an alpha 1 acid glycoprotein. The functions of prealbumin are to transport thyroxine, a task it shares with thyroxine binding globulin, retinol binding protein and vitamin A. Polymorphism of prealbumin is known in the mouse and pig.
Note	Western Blot: Preliminary experiments gave an approx 20-22kDa band in Human Heart, Kidney, Liver, Lung, Placenta and Testis lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 15.9kDa according to NP_000362.1. The 20-22kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Reference Data

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