E2 230K (UBE2O) (C-term) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC
Recommend Dilution	ELISA: 1/32000 (Detection Limit).   Western blot: Preliminary experiments in Human Colon lysates and in lysastes of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry on Paraffin Sections: 4-6 μg/ml.  In paraffin embedded Human Heart shows textured staining in some myocardial fibres.
Reactivity	Bovine, Canine, Human, Mouse, Porcine, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	UBE2O antibody was raised against synthetic peptide (ALLEAGMPECTEDK) from C-terminus of Human UBE2O (NP_071349.3)
Specificity	Recognizes UBE2O (C-term).
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified Ig fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	ubiquitin conjugating enzyme E2 O
Database Link	Entrez Gene 63893 Human UniProt ID Q9C0C9
Synonyms	Ubiquitin-conjugating enzyme E2 O, Ubiquitin-protein ligase O, Ubiquitin carrier protein O, E2-230K, KIAA1734
Reference Data

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