Serotonin transporter (SLC6A4) (605-618) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FC, IF, WB
Recommend Dilution	Peptide ELISA: 1/32000 (Detection Limit). Western blot: 1-3 µg/ml. Approx 75kDa band observed in Mouse, Small Intestine lysates. Immunofluorescence: Strong expression of the protein seen in the Golgi apparatus/vesicles of HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
Reactivity	Bovine, Human, Mouse, Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence, from the C-Terminus of the protein sequence according to NP_001036.1.
Specificity	This antibody recognizes SLC6A4 (605-618).
Formulation	Tris buffered saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer State: Aff - Purified State: Liquid purified Ig fraction
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	solute carrier family 6 member 4
Database Link	Entrez Gene 6532 Human UniProt ID P31645
Background	SERT is a high affinity Na+, Cl- dependent transporter that clears serotonin from synaptic spaces to presynaptic neurons. It has been observed to migrate as 76kDa from rat brain, due to postranslational modifications other bands are observed from different tissues.
Synonyms	5HT transporter, 5HTT, HTT, Sodium-dependent serotonin transporter
Note	Calculated Molecular Weight: 70.3kDa (Human NP_001036.1) and 70.0kDa (Mouse NP_034614.2).
Reference Data

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