MAP2 Chicken Polyclonal Antibody

Specifications

Product Data
Applications	IF, IHC, WB
Recommend Dilution	Western Blot. Immunocytochemistry. Immunohistochemistry. Recommended Dilutions: 1/2000-1/5000 for Western blots. 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Quality Control: This antibody was analyzed by Immunohistochemistry (at a dilution of 1/2000) using Fluorescein-labeled Goat anti-Chicken IgY (1/500 dilution, Cat.-No AP31795FC-N) as the secondary reagent.
Reactivity	Human, Mouse
Host	Chicken
Clonality	Polyclonal
Immunogen	Two synthetic peptides KLH conjugated corresponding to different regions of the MAP-2 gene product, but are shared between the Human (NP_002365) and Mouse (P20357) sequences. Production: After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Specificity	Recognizes MAP-2 (Microtubule-Associated Protein-2).
Formulation	10mM PBS, pH 7.2 containing 0.02% Sodium Azide as preservative. State: Aff - Purified State: Liquid purified (filter sterilized) IgY fraction.
Concentration	lot specific
Purification	Affinity Chromatography using a peptide column.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted in the dark at 2-8°C.
Gene Name	microtubule associated protein 2
Database Link	Entrez Gene 17756 MouseEntrez Gene 4133 Human UniProt ID P11137
Background	Human MAP-2 is a 199,296 dalton protein (1827 amino acids) expressed in neurons of the PNS and CNS, where it serves as a major component of the neuronal cytoskeleton. MAP-2 contributes to structural integrity and cell shape. This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The exact function of this gene is still unknown. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development.
Synonyms	Microtubule-associated protein 2, MAP2, Neuronal Marker
Reference Data
Protein Families	Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS

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