Tnni3 pSer23/24 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/1000.
Reactivity	Human, Mouse, Primate, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser 23/24 of mouse troponin I, cardiac (cTnI)
Specificity	Specific for the ~25k cardiac troponin I protein phosphorylated at Ser 23/24.
Formulation	10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol State: Aff - Purified State: Liquid Ig fraction
Purification	Affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns
Conjugation	Unconjugated
Storage Condition	Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing.
Gene Name	troponin I, cardiac 3
Database Link	Entrez Gene 21954 Mouse UniProt ID P48787
Background	Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place, and wh en cTnI is phosphorylated by protein kinase C and protein kinase A at Ser 23/24 it causes regulation of Ca 2+ - stimulated ATPase (Noland et al, 1995). Evidence suggests that phosphorylation of both se rines 23 and 24 is required for the reduction in Ca 2+ - sensitivity and beneficial for relaxation of the heart (Kooij et al, 2010). Ser 23/24 phosphorylation is important for enhanced relaxation in response to prol onged activation of protein kinase C by endothelin in intact myocytes, while Thr 144 plays an important role in the acute acceleration of relaxation (Westfall et al, 2005).
Synonyms	TNNI3, TNNC1
Reference Data

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