Tnni3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	WB
Recommend Dilution	Western blot: 1/2000 (although higher dilutions can sometimes be used as cardiac troponin I expression is quite high in cardiac muscle).
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Fusion protein of the mouse cardiac troponin I holoprotein
Specificity	Specific for the ~25k cardiac troponin I protein.
Formulation	State: Serum State: Liquid unpurified neat serum
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	troponin I, cardiac 3
Database Link	Entrez Gene 21954 Mouse UniProt ID P48787
Background	Troponin I (cTnI) is 1 of 3 subunits, along with troponin C (TnC) and troponin T (TnT) of troponin complex found in cardiac muscle. cTnI binds to actin in thin myofilaments to hold the troponin-tropomyosin complex in place. Phosphorylation of cardiac isoform of TnI at serines 22,23 in the unique amino-terminal end molecule decreases the ca lcium sensitivity of the sarcomere, promotes calcium dissociation from troponin C and by ext ension enhances rates of cross-bridge cycling and diastolic relaxation (Noland, Jr. et al., 1995; Noland et al., 1989). In addition, studies using reconstituted fibers and mutational analysis have shown that PKC phosp horylation of TnI (largely at Ser43) inhibits the actin-cross bridge reaction and reduces the Ca ++ dependent actomyosin ATPase rate as well as the calcium sensitivity of force generation (Noland, Jr. and Kuo, 1991). Phosphorylation at Thr144 (mediated by several PKC isoforms) reduces maximal tension development and cross-bridge cycling rates (Sumandea et al., 2008). Importantly, changes in th e phosphorylation at each of these sites have been shown to be stage-specific with regard to cardiac disease progression (Walker et al., 2010).
Synonyms	TNNI3, TNNC1
Reference Data

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